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Activity 5.2 — Variegated Leaf & Chlorophyll
NCERT Activity 5.2: Test a variegated leaf with iodine — only the green (chlorophyll) patches turn blue-black, proving chlorophyll is essential for photosynthesis.
How To Perform
1
Expose Plant to Sunlight
Let the variegated leaf plant photosynthesize in sunlight.
2
Boil & Decolorize
Boil the leaf in water then alcohol to remove chlorophyll.
3
Add Iodine Solution
Apply iodine and observe which patches turn blue-black.
4
Conclude
Only green patches turn blue-black — chlorophyll is essential.
⚠️ Safety Notes
- •Alcohol is flammable — always use a water bath for heating.
- •Handle iodine solution with care — it can stain skin and clothing.